• Bacteria and fungi in acute cholecystitis. A prospective study comparing next generation sequencing to culture 

      Dyrhovden, Ruben; Øvrebø, Kjell Kåre; Nordahl, Magnus Vie; Nygaard, Randi M.; Ulvestad, Elling; Kommedal, Øyvind (Peer reviewed; Journal article, 2020)
      Objectives: Guidelines for antibiotic treatment of acute cholecystitis are based on studies using culture techniques for microbial identification. Microbial culture has well described limitations and more comprehensive ...
    • The bacterial aetiology of pleural empyema. A descriptive and comparative metagenomic study 

      Dyrhovden, Ruben; Nygaard, RM; Patel, R; Ulvestad, Elling; Kommedal, Øyvind (Peer reviewed; Journal article, 2019)
      Objectives: The view of pleural empyema as a complication of bacterial pneumonia is changing because many patients lack evidence of underlying pneumonia. To further our understanding of pathophysiological mechanisms, we ...
    • Escherichia marmotae—a Human Pathogen Easily Misidentified as Escherichia coli 

      Sivertsen, Audun; Dyrhovden, Ruben; Tellevik, Marit Gjerde; Bruvold, Torbjørn Sæle; Nybakken, Eirik Jovall; Skutlaberg, Dag Harald; Skarstein, Ingerid; Kommedal, Øyvind (Journal article; Peer reviewed, 2022)
      We hereby present the first descriptions of human-invasive infections caused by Escherichia marmotae, a recently described species that encompasses the former “Escherichia cryptic clade V.” We describe four cases, one acute ...
    • Investigating the human jejunal microbiota 

      Villmones, Heidi Cecilie; Svanevik, Marius; Ulvestad, Elling; Stenstad, Tore; Anthonisen, Inger Lill; Nygaard, Randi Monsen; Dyrhovden, Ruben; Kommedal, Øyvind (Journal article; Peer reviewed, 2022)
      Descriptions of the small intestinal microbiota are deficient and conflicting. We aimed to get a reliable description of the jejunal bacterial microbiota by investigating samples from two separate jejunal segments collected ...
    • Managing Contamination and Diverse Bacterial Loads in 16S rRNA Deep Sequencing of Clinical Samples: Implications of the Law of Small Numbers 

      Dyrhovden, Ruben; Rippin, Martin; Øvrebø, Kjell Kåre; Nygaard, Randi Monsen; Ulvestad, Elling; Kommedal, Øyvind (Journal article; Peer reviewed, 2021)
      In this article, we investigate patterns of microbial DNA contamination in targeted 16S rRNA amplicon sequencing (16S deep sequencing) and demonstrate how this can be used to filter background bacterial DNA in diagnostic ...
    • Persistent Fever and Positive PCR 90 Days Post-SARS-CoV-2 Infection in a Rituximab-Treated Patient: A Case of Late Antiviral Treatment 

      Ertesvåg, Nina Urke; Sakkestad, Sunniva Todnem; Zhou, Fan; Hoff, Ingrid; Kristiansen, Trygve; Jonassen, Trygve Müller; Follesø, Elisabeth; Brokstad, Karl Albert; Dyrhovden, Ruben; Mohn, Kristin Greve-Isdahl (Journal article; Peer reviewed, 2022)
      Background: Persistent fever after SARS-CoV-2 infection in rituximab-treated patients has been reported. Due to reduced sensitivity in conventional sampling methods and unspecific symptoms in these patients, distinguishing ...
    • Pleural Empyema Caused by Streptococcus intermedius and Fusobacterium nucleatum: A Distinct Entity of Pleural Infections 

      Dyrhovden, Ruben; Eagan, Tomas Mikal Lind; Fløtten, Øystein; Siljan, William Ward; Leegaard, Truls Michael; Bø, Bjørnar; Fardal, Hilde; Grøvan, Fredrik; Kildahl-Andersen, Arne; Larssen, Kjersti Wik; Tilseth, Rune; Hjetland, Reidar; Løes, Sigbjørn Suk; Lindemark, Frode; Tellevik, Marit Gjerde; Breistein, Rebecca Irene; Kommedal, Øyvind (Journal article; Peer reviewed, 2023)
      Background Many community-acquired pleural infections are caused by facultative and anaerobic bacteria from the human oral microbiota. The epidemiology, clinical characteristics, pathogenesis, and etiology of such ...
    • The use of 16S rRNA targeted next generation sequencing in diagnostics of polymicrobial invasive infections 

      Dyrhovden, Ruben (Doctoral thesis, 2023-08-21)
      16S rRNA dypsekvensering muliggjør, som vist i flere vitenskapelige arbeider, en mer fullstendig karakterisering av komplekse bakterielle mikrobiota enn det som kan oppnås ved tradisjonelle, dyrkningsbaserte, mikrobiologiske ...